PMID:22505047
| Citation |
Gupta, P, Swanberg, JC and Lee, KH A single nucleotide polymorphism in ycdC alters tRNA synthetase expression and results in hypersecretion in Escherichia coli. Biotechnol. Prog. 28:646-53 |
|---|---|
| Abstract |
The most important approach to the development of platform organisms for recombinant protein production relies on random mutagenesis and phenotypic selection. Complex phenotypes, including those associated with significantly elevated expression and secretion of heterologous proteins, are the result of multiple genomic mutations. Using next generation sequencing, a parent and derivative hypersecreter strain (B41) of Escherichia coli were sequenced with an average coverage of 52.8X and 55X, respectively. A new base-pair calling program, revealed a single nucleotide polymorphism in the B41 genome at position 1,074,787, resulting in translation termination near the N-terminus of a transcriptional regulator protein, RutR, coded by the ycdC gene. We verified the hypersecretion phenotype in a ycdC::Tn5 mutant and observed a 3.4-fold increase in active hemolysin secretion, consistent with the increase observed in B41 strain. mRNA expression profiling showed decreased expression of tRNA-synthetases and some amino acid transporters in the ycdC::Tn5 mutant. This study demonstrates the power of next generation sequencing to characterize mutants leading to successful metabolic engineering strategies for strain improvement. |
| Links |
PubMed Online version:10.1002/btpr.1550 |
| Keywords |
Amino Acyl-tRNA Synthetases/genetics; Base Sequence; Blotting, Western; DNA Primers; DNA, Bacterial/genetics; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Polymorphism, Single Nucleotide; RNA, Messenger/genetics; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Nucleic Acid |
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