PMID:22419076
| Citation |
Epstein, RH, Bolle, A and Steinberg, CM (2012) Amber mutants of bacteriophage T4D: their isolation and genetic characterization. Genetics 190:833-40 |
|---|---|
| Abstract |
We have isolated a large number of mutants of bacteriophage T4D that are unable to form plaques on strain B of Escherichia coli, but are able to grow (nearly) normally on some other strains of E. coli, in particular strain CR63. These mutants, designated amber (am), have been characterized by complementation tests, by genetic crosses, and by their response to chemical mutagens. It is concluded that a particular subclass of base substitution mutations may give rise to amber mutants and that such mutants occur in many genes, which are widely distributed over the T4 genome. |
| Links |
PubMed PMC3296251 Online version:10.1534/genetics.112.138438 |
| Keywords |
Alleles; Bacteriophage T4/drug effects; Bacteriophage T4/genetics; Bacteriophage T4/isolation & purification; Chromosome Mapping; Codon, Nonsense/drug effects; Gene Order; Genes, Viral; Genetic Complementation Test |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
This manuscript describes the first identification of conditional nonsense mutations. This work was cited as unpublished for 50 years until the manuscript was found and published in Genetics after the death of Dick Epstein in 2011 (see the introductory note by FW Stahl).
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.