PMID:22295907
| Citation |
Stratmann, T, Pul, Ü, Wurm, R, Wagner, R and Schnetz, K (2012) RcsB-BglJ activates the Escherichia coli leuO gene, encoding an H-NS antagonist and pleiotropic regulator of virulence determinants. Mol. Microbiol. 83:1109-23 |
|---|---|
| Abstract |
The LysR-type transcription factor LeuO is involved in regulation of pathogenicity determinants and stress responses in Enterobacteriaceae, and acts as antagonist of the global repressor H-NS. Expression of the leuO gene is repressed by H-NS, and it is upregulated in stationary phase and under amino acid starvation conditions. Here, we show that the heterodimer of the FixJ/NarL-type transcription regulators RcsB and BglJ strongly activates expression of leuO and that RcsB-BglJ regulates additional loci. Activation of leuO by RcsB-BglJ is independent of the Rcs phosphorelay system. RcsB-BglJ binds to the leuO promoter region and activates one of two leuO promoters mapped in vivo. Moreover, LeuO antagonizes activation of leuO by RcsB-BglJ and acts as negative autoregulator in vivo and in vitro. Further, the H-NS paralogue StpA causes repression of leuO in addition to H-NS. Together, our data suggest a complex arrangement of regulatory elements and they indicate a feedback control mechanism of leuO expression. |
| Links |
PubMed Online version:10.1111/j.1365-2958.2012.07993.x |
| Keywords |
Base Sequence; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/antagonists & inhibitors; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fimbriae Proteins/antagonists & inhibitors; Fimbriae Proteins/genetics; Fimbriae Proteins/metabolism; Gene Expression Regulation, Bacterial; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Molecular Sequence Data; Operon; Promoter Regions, Genetic; Protein Binding; Trans-Activators/genetics; Trans-Activators/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Transcriptional Activation; Virulence Factors/genetics; Virulence Factors/metabolism |
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