PMID:22245966
| Citation |
Malet, H, Canellas, F, Sawa, J, Yan, J, Thalassinos, K, Ehrmann, M, Clausen, T and Saibil, HR (2012) Newly folded substrates inside the molecular cage of the HtrA chaperone DegQ. Nat. Struct. Mol. Biol. 19:152-7 |
|---|---|
| Abstract |
The HtrA protein family combines chaperone and protease activities and is essential for protein quality control in many organisms. Whereas the mechanisms underlying the proteolytic function of HtrA proteins are well characterized, their chaperone activity remains poorly understood. Here we describe cryo-EM structures of Escherichia coli DegQ in its 12- and 24-mer states in complex with model substrates, providing a structural model of HtrA chaperone action. Up to six lysozyme substrates bind inside the DegQ 12-mer cage and are visualized in a close-to-native state. An asymmetric reconstruction reveals the binding of a well-ordered lysozyme to four DegQ protomers. DegQ PDZ domains are located adjacent to substrate density and their presence is required for chaperone activity. The substrate-interacting regions appear conserved in 12- and 24-mer cages, suggesting a common mechanism of chaperone function. |
| Links |
PubMed PMC3272482 Online version:10.1038/nsmb.2210 |
| Keywords |
Cryoelectron Microscopy; Escherichia coli/enzymology; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Escherichia coli Proteins/ultrastructure; Models, Molecular; Molecular Chaperones/chemistry; Molecular Chaperones/metabolism; Molecular Chaperones/ultrastructure; Muramidase/chemistry; Muramidase/metabolism; Protein Multimerization; Protein Structure, Quaternary; Serine Endopeptidases/chemistry; Serine Endopeptidases/metabolism; Serine Endopeptidases/ultrastructure |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.