PMID:22151946
| Citation |
Nannenga, BL and Baneyx, F (2011) Enhanced expression of membrane proteins in E. coli with a P(BAD) promoter mutant: synergies with chaperone pathway engineering strategies. Microb. Cell Fact. 10:105 |
|---|---|
| Abstract |
Membrane proteins (MPs) populate 20-30% of genomes sequenced to date and hold potential as therapeutic targets as well as for practical applications in bionanotechnology. However, MP toxicity and low yields in normally robust expression hosts such as E. coli has curtailed progress in our understanding of their structure and function. |
| Links |
PubMed PMC3265434 Online version:10.1186/1475-2859-10-105 |
| Keywords |
Archaeal Proteins/genetics; Archaeal Proteins/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Halobacteriaceae/genetics; Membrane Proteins/genetics; Membrane Proteins/metabolism; Metabolic Engineering; Molecular Chaperones/genetics; Molecular Chaperones/metabolism; Operon; Peptidylprolyl Isomerase/genetics; Peptidylprolyl Isomerase/metabolism; Point Mutation; Promoter Regions, Genetic; Response Elements; Rhodopsin/genetics; Rhodopsin/metabolism |
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