PMID:22144354
| Citation |
Meynial-Salles, I and Soucaille, P (2012) Creation of new metabolic pathways or improvement of existing metabolic enzymes by in vivo evolution in Escherichia coli. Methods Mol. Biol. 834:75-86 |
|---|---|
| Abstract |
A method for in vivo evolution of metabolic pathways in bacteria is described. This method is a powerful tool for synthetic biology type of metabolic design and can lead to the creation of new metabolic pathways or the improvement of existing metabolic enzymes. The proposed strategy also permits to relate the evolved phenotype to the genotype and to analyze evolution phenomenon at the genetic, biochemical, and metabolic levels. |
| Links |
PubMed Online version:10.1007/978-1-61779-483-4_6 |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Evolution, Molecular; Gene Expression; Gene Order; Metabolic Engineering/methods; Metabolic Networks and Pathways; Mutation; Phenotype; Plasmids/genetics |
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