PMID:22144352
| Citation |
Suh, W (2012) High isoprenoid flux Escherichia coli as a host for carotenoids production. Methods Mol. Biol. 834:49-62 |
|---|---|
| Abstract |
A noncarotenogenic microbe E. coli was engineered for high production of carotenoids. To increase the isoprenoid flux, the chromosomal native promoters of the rate-controlling steps (dxs, idi and ispDispF) in the isoprenoid pathway were replaced with a strong bacteriophage T5 promoter (P(T5)) by using the λ-Red recombinase system in combination with the Flp/FRT site-specific recombination system for marker excision and P1 transduction for gene trait stacking. The resulting high isoprenoid flux E. coli can be used as a starting strain to produce various carotenoids by introducing heterologous carotenoid genes. In this study, the high isoprenoid flux E. coli was transformed with a plasmid carrying the β-carotene biosynthetic genes from Pantoea stewartii for β-carotene production. |
| Links |
PubMed Online version:10.1007/978-1-61779-483-4_4 |
| Keywords |
Carotenoids/biosynthesis; Chromosomes, Bacterial; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression Regulation, Bacterial; Gene Order; Genetic Engineering/methods; Plasmids/genetics; Promoter Regions, Genetic; Recombination, Genetic; Terpenes/metabolism; Transduction, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.