PMID:2213883

Citation	Waldburger, C, Gardella, T, Wong, R and Susskind, MM (1990) Changes in conserved region 2 of Escherichia coli sigma 70 affecting promoter recognition. J. Mol. Biol. 215:267-76
Abstract	We describe a mutation in rpoD, the gene encoding the sigma 70 subunit of RNA polymerase, which alters the promoter specificity of the holoenzyme in vivo. The mutant sigma causes a substantial and specific increase in the activity of mutant ant and lac promoters with a T.A to C.G substitution at position -12, the first position of the -10 hexamer. The rpoD mutation is a single base-pair substitution causing a Gln----His change at position 437, which is in a domain of conserved region 2.4 that is predicted to form an alpha-helix. Gln437 would lie one turn of the alpha-helix away from Thr440, which was previously implicated in recognition of position -12. The rpoD-QH437 mutation described here lends further support to the model that region 2.4 of sigma is involved in recognition of the 5' end of the -10 hexamer. In addition, two rpoD mutations with non-specific effects on promoter recognition are described.
Links	PubMed
Keywords	Amino Acid Sequence; Chromosome Mapping; DNA Mutational Analysis; DNA-Binding Proteins/ultrastructure; DNA-Directed RNA Polymerases/chemistry; DNA-Directed RNA Polymerases/genetics; Escherichia coli/genetics; Molecular Sequence Data; Promoter Regions, Genetic; Protein Conformation; Recombinant Proteins/chemistry; Recombinant Proteins/ultrastructure; Regulatory Sequences, Nucleic Acid; Sigma Factor/physiology; Substrate Specificity; Transcription, Genetic
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