PMID:22121193
| Citation |
Mavridou, DA, Stevens, JM, Mönkemeyer, L, Daltrop, O, di Gleria, K, Kessler, BM, Ferguson, SJ and Allen, JW (2012) A pivotal heme-transfer reaction intermediate in cytochrome c biogenesis. J. Biol. Chem. 287:2342-52 |
|---|---|
| Abstract |
c-Type cytochromes are widespread proteins, fundamental for respiration or photosynthesis in most cells. They contain heme covalently bound to protein in a highly conserved, highly stereospecific post-translational modification. In many bacteria, mitochondria, and archaea this heme attachment is catalyzed by the cytochrome c maturation (Ccm) proteins. Here we identify and characterize a covalent, ternary complex between the heme chaperone CcmE, heme, and cytochrome c. Formation of the complex from holo-CcmE occurs in vivo and in vitro and involves the specific heme-binding residues of both CcmE and apocytochrome c. The enhancement and attenuation of the amounts of this complex correlates completely with known consequences of mutations in genes for other Ccm proteins. We propose the complex is a trapped catalytic intermediate in the cytochrome c biogenesis process, at the point of heme transfer from CcmE to the cytochrome, the key step in the maturation pathway. |
| Links |
PubMed PMC3268396 Online version:10.1074/jbc.M111.313692 |
| Keywords |
Bacterial Outer Membrane Proteins/biosynthesis; Bacterial Outer Membrane Proteins/genetics; Cytochromes c/biosynthesis; Cytochromes c/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/genetics; Heme/genetics; Heme/metabolism; Hemeproteins/biosynthesis; Hemeproteins/genetics; Multiprotein Complexes/genetics; Multiprotein Complexes/metabolism; Protein Biosynthesis/physiology |
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