PMID:22101847
| Citation |
Skoog, K, Söderström, B, Widengren, J, von Heijne, G and Daley, DO (2012) Sequential closure of the cytoplasm and then the periplasm during cell division in Escherichia coli. J. Bacteriol. 194:584-6 |
|---|---|
| Abstract |
To visualize the latter stages of cell division in live Escherichia coli, we have carried out fluorescence recovery after photobleaching (FRAP) on 121 cells expressing cytoplasmic green fluorescent protein and periplasmic mCherry. Our data show conclusively that the cytoplasm is sealed prior to the periplasm during the division event. |
| Links |
PubMed PMC3264074 Online version:10.1128/JB.06091-11 |
| Keywords |
Cell Division; Cytoplasm/genetics; Cytoplasm/metabolism; Escherichia coli/chemistry; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Fluorescence Recovery After Photobleaching; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Periplasm/genetics; Periplasm/metabolism; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism |
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