PMID:22095389
| Citation |
Trchounian, K, Pinske, C, Sawers, RG and Trchounian, A (2012) Characterization of Escherichia coli [NiFe]-hydrogenase distribution during fermentative growth at different pHs. Cell Biochem. Biophys. 62:433-40 |
|---|---|
| Abstract |
The contribution made by each of the three active [NiFe]-hydrogenases (Hyd) of Escherichia coli during fermentation of glucose or glycerol in peptone-based medium at different pHs was analysed. The activities of the hydrogen-oxidizing Hyd-1 and Hyd-2 enzymes showed a reciprocal dependence on the pH of the medium while Hyd-3, a key component of the hydrogen-evolving formate hydrogenlyase complex, was mainly active at pH 6.5. Our findings identify the conditions during fermentation of glucose or glycerol under which each [NiFe]-hydrogenase is optimally active and demonstrate a previously unrecognized dependence on Hyd-1 activity at low pH. |
| Links |
PubMed Online version:10.1007/s12013-011-9325-y |
| Keywords |
Escherichia coli/drug effects; Escherichia coli/enzymology; Fermentation; Glucose/pharmacology; Glycerol/pharmacology; Hydrogen-Ion Concentration; Hydrogenase/metabolism; Oxidoreductases/metabolism; Protein Isoforms/metabolism |
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