PMID:22092684
| Citation |
Yamamoto, K, Oshima, T, Nonaka, G, Ito, H and Ishihama, A (2011) Induction of the Escherichia coli cysK gene by genetic and environmental factors. FEMS Microbiol. Lett. 323:88-95 |
|---|---|
| Abstract |
Cysteine synthase A encoded by cysK catalyzes the synthesis of cysteine from O-acetylserine. Expression of cysK in Escherichia coli is under the control of CysB, a LysR family transcription factor. Herein we showed that the expression of cysK is regulated by several genetic and environmental factors in addition to CysB: two genetic factors, OmpR and CysE, and lithium. Based on the findings, we constructed the high-level expression system of cysK. |
| Links |
PubMed Online version:10.1111/j.1574-6968.2011.02364.x |
| Keywords |
Bacterial Proteins/metabolism; Cysteine Synthase/biosynthesis; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins/biosynthesis; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Lithium/metabolism; Serine O-Acetyltransferase/metabolism; Trans-Activators/metabolism; Transcriptional Activation |
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