PMID:22081388
| Citation |
Freddolino, PL, Amini, S and Tavazoie, S (2012) Newly identified genetic variations in common Escherichia coli MG1655 stock cultures. J. Bacteriol. 194:303-6 |
|---|---|
| Abstract |
We have recently identified seven mutations in commonly used stocks of the sequenced Escherichia coli strain MG1655 which do not appear in the reference sequence. The mutations are likely to cause loss of function of the glpR and crl genes, which may have serious implications for physiological experiments using the affected strains. |
| Links |
PubMed PMC3256642 Online version:10.1128/JB.06087-11 |
| Keywords |
Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; DNA-Directed RNA Polymerases/metabolism; Escherichia coli K12/classification; Escherichia coli K12/genetics; Escherichia coli K12/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Profiling; Gene Expression Regulation, Bacterial/physiology; Genetic Variation; Glycerophosphates/metabolism; Mutation; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sigma Factor/metabolism |
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