PMID:2207165
| Citation |
Moine, H, Ehresmann, B, Romby, P, Ebel, JP, Grunberg-Manago, M, Springer, M and Ehresmann, C (1990) The translational regulation of threonyl-tRNA synthetase. Functional relationship between the enzyme, the cognate tRNA and the ribosome. Biochim. Biophys. Acta 1050:343-50 |
|---|---|
| Abstract |
The E. coli threonyl-tRNA synthetase gene is negatively autoregulated at the translational level by a direct binding of the enzyme to the leader region of the thrS mRNA. This region folds in four well-defined domains. The enzyme binds to the leader at two major sites: the first is a stem-loop structure located in domain II upstream of the translational initiation site (domain I) which shares structural analogies with the anticodon arm of several tRNA(Thr) isoacceptors. The second site corresponds to a stable stem-loop structure located in domain IV. Both sites are separated by a large unpaired region (domain III). In vivo and in vitro experiments show that the structural integrity of both sites is required for the regulatory process. The binding of the enzyme to its mRNA target site represses its translation by preventing the ribosome from binding to its attachment site. tRNA(Thr) suppresses this inhibitory effect by displacing the mRNA from the enzyme at both the upstream stem-loop structure and the tRNA-like anticodon arm. |
| Links | |
| Keywords |
Anticodon/genetics; Base Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Genes, Bacterial; Models, Molecular; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Protein Biosynthesis; RNA, Messenger/genetics; RNA, Transfer, Thr/genetics; RNA, Transfer, Thr/metabolism; Ribosomes/metabolism; Threonine-tRNA Ligase/genetics |
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