PMID:2205514
| Citation |
Abdurashidova, GG, Tsvetkova, EA and Budowsky, EI (1990) Determination of tRNA nucleotide residues directly interacting with proteins in the post- and pretranslocated ribosomal complexes. FEBS Lett. 269:398-401 |
|---|---|
| Abstract |
Nucleotide residues of E. coli tRNA interacting directly with proteins in pre- and posttranslocated ribosomal complexes have been identified by analysis of photo-induced tRNA-protein cross-links. A9, G18, A26 and U59 residues of NAcPhePhe-tRNA, located in the Ab-site (pretranslocated complex) have been cross-linked with proteins S10, L27, S7 and L2 respectively. In deacylated tRNA, located in the Pb-site, residues C17, G44, C56 and U60 have been cross-linked with proteins L2, L5, L27 and S9 respectively. The G44-L5 cross-link disappeared after translocation (NAc-PhePhe-tRNA located in the Pt-site). |
| Links | |
| Keywords |
Base Sequence; Escherichia coli/genetics; Escherichia coli/metabolism; Models, Structural; Molecular Sequence Data; Nucleic Acid Conformation; Protein Binding; RNA Processing, Post-Transcriptional; RNA, Transfer/genetics; RNA, Transfer/metabolism; Ribosomal Proteins/metabolism; Ribosomes/metabolism |
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