PMID:22033919

Citation	Lycklama a Nijeholt, JA, Wu, ZC and Driessen, AJ (2011) Conformational dynamics of the plug domain of the SecYEG protein-conducting channel. J. Biol. Chem. 286:43881-90
Abstract	The central pore of the SecYEG preprotein-conducting channel is closed at the periplasmic face of the membrane by a plug domain. To study its conformational dynamics, the plug was labeled site-specifically with an environment-sensitive fluorophore. In the presence of a stable preprotein translocation inter-mediate, the SecY plug showed an enhanced solvent exposure consistent with a displacement from the hydrophobic central pore region. In contrast, binding and insertion of a ribosome-bound nascent membrane protein did not alter the plug conformation. These data indicate different plug dynamics depending on the ligand bound state of the SecYEG channel.
Links	PubMed PMC3243504 Online version:10.1074/jbc.M111.297507
Keywords	Cell Membrane/metabolism; Crystallography, X-Ray/methods; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Fluorescent Dyes/pharmacology; Hydrophobic and Hydrophilic Interactions; Methanococcus/metabolism; Models, Molecular; Molecular Conformation; Plasmids/metabolism; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Ribosomes/metabolism
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