PMID:22026739
| Citation |
Hu, Y, Benedik, MJ and Wood, TK (2012) Antitoxin DinJ influences the general stress response through transcript stabilizer CspE. Environ. Microbiol. 14:669-79 |
|---|---|
| Abstract |
Antitoxins are becoming recognized as proteins that regulate more than their own synthesis; for example, we found previously that antitoxin MqsA of the Escherichia coli toxin/antitoxin (TA) pair MqsR/MqsA directly represses the gene encoding the stationary-phase sigma factor RpoS. Here, we investigated the physiological role of antitoxin DinJ of the YafQ/DinJ TA pair and found DinJ also affects the general stress response by decreasing RpoS levels. Corroborating the reduced RpoS levels upon producing DinJ, the RpoS-regulated phenotypes of catalase activity, cell adhesins and cyclic diguanylate decreased while swimming increased. Using a transcriptome search and DNA-binding assays, we determined that the mechanism by which DinJ reduces RpoS is by repressing cspE at the LexA palindrome; cold-shock protein CspE enhances translation of rpoS mRNA. Inactivation of CspE abolishes the ability of DinJ to influence RpoS. Hence, DinJ influences the general stress response indirectly by regulating cspE. |
| Links |
PubMed Online version:10.1111/j.1462-2920.2011.02618.x |
| Keywords |
Antitoxins/genetics; Antitoxins/metabolism; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Catalase/metabolism; Cell Adhesion/physiology; Cell Movement/physiology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/physiology; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; RNA, Messenger/metabolism; Sigma Factor/genetics; Sigma Factor/metabolism; Stress, Physiological/physiology; Transcriptome |
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