PMID:22026583

Citation	Hensley, MP, Tierney, DL and Crowder, MW (2011) Zn(II) binding to Escherichia coli 70S ribosomes. Biochemistry 50:9937-9
Abstract	Escherichia coli 70S ribosomes tightly bind 8 equiv of Zn(II), and EXAFS spectra indicate that Zn(II) may be protein-bound. Ribosomes were incubated with EDTA and Zn(II), and after dialysis, the resulting ribosomes bound 5 and 11 equiv of Zn(II), respectively. EXAFS studies show that the additional Zn(II) in the zinc-supplemented ribosomes binds in part to the phosphate backbone of the ribosome. Lastly, in vitro translation studies demonstrate that EDTA-treated ribosomes do not synthesize an active Zn(II)-bound metalloenzyme, while the as-isolated ribosomes do. These studies demonstrate that the majority of intracellular Zn(II) resides in the ribosome.
Links	PubMed PMC3220600 Online version:10.1021/bi200619w
Keywords	Binding Sites; Escherichia coli/metabolism; Escherichia coli Proteins/metabolism; Metalloproteins/metabolism; Models, Molecular; Phosphates/metabolism; Ribosomal Proteins/metabolism; Ribosome Subunits, Large, Bacterial/metabolism; Ribosome Subunits, Small, Bacterial/metabolism; Zinc/metabolism
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