PMID:2198274
| Citation |
Wang, HC and Gayda, RC (1990) High-level expression of the FtsA protein inhibits cell septation in Escherichia coli K-12. J. Bacteriol. 172:4736-40 |
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| Abstract |
DNA fragments encoding the ftsA gene were subcloned into plasmids downstream of a lac promoter or a tac promoter. These plasmid constructs, when transformed into wild-type and mutant strains, inhibited normal cell septation, causing the formation of long nonseptate filaments. This phenotype is due to overproduction of the FtsA protein. |
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| Keywords |
Bacterial Proteins/genetics; Cell Division; Cloning, Molecular; Escherichia coli/cytology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression; Genes, Bacterial; Isopropyl Thiogalactoside/pharmacology; Mutation; Plasmids; Restriction Mapping |
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