PMID:2198273
| Citation |
Leclerc, G, Noël, G and Drapeau, GR (1990) Molecular cloning, nucleotide sequence, and expression of shl, a new gene in the 2-minute region of the genetic map of Escherichia coli. J. Bacteriol. 172:4696-700 |
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| Abstract |
Cells of Escherichia coli that harbor supH (an allele of the wild-type gene serU) are sensitive to UV irradiation and temperature and appear to have an impaired cell division control mechanism. We found that a gene located at the 2-min region, designated shl, inhibited the growth of supH-harboring cells when carried by a high-copy-number plasmid, whereas the same plasmid had no visible effect when present in parental cells. The amino acid sequence predicted from the nucleotide sequence of the shl gene indicated a similarity to the GalR and LacI repressor proteins, suggesting it is a transcription regulator. The sequence between the promoter and the structural genes revealed the presence of a short open reading frame of 28 amino acid residues followed by a segment of 81 base pairs. These structural features suggest that a transcription antitermination mechanism may be involved in the regulation of expression of the shl gene. The possibility that shl is a regulator of serU is discussed. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cell Division; Cloning, Molecular; Escherichia coli/genetics; Escherichia coli/growth & development; Gene Expression; Genes, Bacterial; Molecular Sequence Data; Protein Sorting Signals/genetics; Repressor Proteins/genetics; Restriction Mapping; Sequence Homology, Nucleic Acid; Transcription Factors/genetics |
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