PMID:21980523
| Citation |
Mika, JT, Krasnikov, V, van den Bogaart, G, de Haan, F and Poolman, B (2011) Evaluation of pulsed-FRAP and conventional-FRAP for determination of protein mobility in prokaryotic cells. PLoS ONE 6:e25664 |
|---|---|
| Abstract |
Macromolecule mobility is often quantified with Fluorescence Recovery After Photobleaching (FRAP). Throughout literature a wide range of diffusion coefficients for GFP in the cytoplasm of Escherichia coli (3 to 14 µm²/s) is reported using FRAP-based approaches. In this study, we have evaluated two of these methods: pulsed-FRAP and "conventional"-FRAP. |
| Links |
PubMed PMC3182251 Online version:10.1371/journal.pone.0025664 |
| Keywords |
Cytoplasm/metabolism; Diffusion; Escherichia coli K12/cytology; Escherichia coli Proteins/metabolism; Fluorescence Recovery After Photobleaching/methods; Green Fluorescent Proteins/metabolism; Movement; Osmotic Pressure |
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