PMID:21943062
| Citation |
Ploss, M and Kuhn, A (2011) Membrane insertion and assembly of epitope-tagged gp9 at the tip of the M13 phage. BMC Microbiol. 11:211 |
|---|---|
| Abstract |
Filamentous M13 phage extrude from infected Escherichia coli with a tip structure composed of gp7 and gp9. This tip structure is extended by the assembly of the filament composed of the major coat protein gp8. Finally, gp3 and gp6 terminate the phage structure at the proximal end. Up to now, gp3 has been the primary tool for phage display technology. However, gp7, gp8 and gp9 could also be used for phage display and these phage particles should bind to two different or more surfaces when the modified coat proteins are combined. Therefore, we tested here if the amino-terminal end of gp9 can be modified and whether the modified portion is exposed and detectable on the M13 phage particles. |
| Links |
PubMed PMC3193035 Online version:10.1186/1471-2180-11-211 |
| Keywords |
Amino Acid Motifs; Amino Acid Sequence; Bacteriophage M13/chemistry; Bacteriophage M13/genetics; Bacteriophage M13/physiology; Capsid Proteins/chemistry; Capsid Proteins/genetics; Capsid Proteins/metabolism; Cell Membrane/virology; Epitopes/chemistry; Epitopes/genetics; Epitopes/metabolism; Escherichia coli/virology; Molecular Sequence Data; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Virus Assembly |
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