PMID:2193911
| Citation |
Halling, C and Calendar, R (1990) Bacteriophage P2 ogr and P4 delta genes act independently and are essential for P4 multiplication. J. Bacteriol. 172:3549-58 |
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| Abstract |
Satellite bacteriophage P4 requires the products of the late genes of a helper phage such as P2 for lytic growth. Expression of the P2 late genes is positively regulated by the P2 ogr gene in a process requiring P2 DNA replication. Transactivation of P2 late gene expression by P4 requires the P4 delta gene product and works even in the absence of P2 DNA replication. We have made null mutants of the P2 ogr and P4 delta genes. In the absence of the P4 delta gene product, P4 multiplication required both the P2 ogr protein and P2 DNA replication. In the absence of the P2 ogr gene product, P4 multiplication required the P4 delta protein. In complementation experiments, we found that the P2 ogr protein was made in the absence of P2 DNA replication but could not function unless P2 DNA replicated. We produced P4 delta protein from a plasmid and found that it complemented the null P4 delta and P2 ogr mutants. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Coliphages/genetics; Coliphages/physiology; Escherichia coli/genetics; Genes, Viral; Genotype; Helper Viruses/genetics; Helper Viruses/physiology; Molecular Sequence Data; Phenotype; Plasmids; Restriction Mapping; Temperature; Virus Replication/genetics |
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