PMID:2192230
| Citation |
Flannigan, KA, Hennigan, SH, Vogelbacker, HH, Gots, JS and Smith, JM (1990) Purine biosynthesis in Escherichia coli K12: structure and DNA sequence studies of the purHD locus. Mol. Microbiol. 4:381-92 |
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| Abstract |
The de novo purine biosynthetic enzymes 5-amino-4-imidazolecarboxamide-ribonucleotide (AICAR) transformylase (EC 2.1.2.3), IMP cyclohydrolase (EC 3.5.4.10) and glycineamide-ribonucleotide (GAR) synthetase (EC 2.1.2.2) are encoded by the purHD locus of Escherichia coli. The DNA sequence of this locus revealed two open reading frames encoding polypeptides of Mr 57,335 and 45,945 (GAR synthetase), respectively, that formed an operon. The DNA sequence, maxicell and complementation analyses all supported the concept that the Mr 57,335 polypeptide is the product of the purH gene and encodes a bifunctional protein containing both AICAR transformylase and IMP cyclohydrolase activities. The 5' end of the purHD mRNA was determined by primer extension mapping and contains two regions of dyad symmetry capable of forming 'hairpin' loops where the formation of the one would prevent the formation of the other but not vice versa. Regulation by the purR gene product was explained by the discovery of a purR binding site in the purHD control region. |
| Links | |
| Keywords |
Acyltransferases/biosynthesis; Acyltransferases/genetics; Amino Acid Sequence; Base Sequence; Carbon-Nitrogen Ligases; Cloning, Molecular; DNA, Bacterial/analysis; Escherichia coli/enzymology; Escherichia coli/genetics; Humans; Hydroxymethyl and Formyl Transferases; Ligases/genetics; Molecular Sequence Data; Nucleic Acid Conformation; Nucleotide Deaminases/biosynthesis; Nucleotide Deaminases/genetics; Phosphoribosylaminoimidazolecarboxamide Formyltransferase; Restriction Mapping; Salmonella typhimurium/enzymology; Salmonella typhimurium/genetics |
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