PMID:2190985
| Citation |
Hamm-Alvarez, SF, Sancar, A and Rajagopalan, KV (1990) The presence and distribution of reduced folates in Escherichia coli dihydrofolate reductase mutants. J. Biol. Chem. 265:9850-6 |
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| Abstract |
Escherichia coli DNA photolyase was overproduced and purified from each of two mutant E. coli strains lacking dihydrofolate reductase. The extent of over-production in the mutants was comparable to that seen in the wild type strain. Examination of the isolated photolyase from these strains revealed that the folate cofactor, 5,10-methenyltetrahydrofolate, was present in these proteins at a level of 60-80% compared to that purified from the wild type strain. Further examination of the dihydrofolate reductase-deficient strains revealed the presence of other tetrahydrofolate derivatives. These findings demonstrate that dihydrofolate reductase is not essential for the production of tetrahydrofolates in E. coli. |
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| Keywords |
Chromatography, High Pressure Liquid; Deoxyribodipyrimidine Photo-Lyase/isolation & purification; Deoxyribodipyrimidine Photo-Lyase/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Folic Acid/analogs & derivatives; Folic Acid/isolation & purification; Folic Acid/metabolism; Genotype; Mutation; Tetrahydrofolate Dehydrogenase/genetics |
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