PMID:21903814
| Citation |
Wang, W, Li, GW, Chen, C, Xie, XS and Zhuang, X (2011) Chromosome organization by a nucleoid-associated protein in live bacteria. Science 333:1445-9 |
|---|---|
| Abstract |
Bacterial chromosomes are confined in submicrometer-sized nucleoids. Chromosome organization is facilitated by nucleoid-associated proteins (NAPs), but the mechanisms of action remain elusive. In this work, we used super-resolution fluorescence microscopy, in combination with a chromosome-conformation capture assay, to study the distributions of major NAPs in live Escherichia coli cells. Four NAPs--HU, Fis, IHF, and StpA--were largely scattered throughout the nucleoid. In contrast, H-NS, a global transcriptional silencer, formed two compact clusters per chromosome, driven by oligomerization of DNA-bound H-NS through interactions mediated by the amino-terminal domain of the protein. H-NS sequestered the regulated operons into these clusters and juxtaposed numerous DNA segments broadly distributed throughout the chromosome. Deleting H-NS led to substantial chromosome reorganization. These observations demonstrate that H-NS plays a key role in global chromosome organization in bacteria. |
| Links |
PubMed PMC3329943 Online version:10.1126/science.1204697 |
| Keywords |
Binding Sites; Cell Division; Chromosomes, Bacterial/metabolism; Chromosomes, Bacterial/ultrastructure; DNA, Bacterial/chemistry; DNA, Bacterial/metabolism; DNA-Binding Proteins/metabolism; Escherichia coli K12/genetics; Escherichia coli K12/metabolism; Escherichia coli K12/ultrastructure; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Factor For Inversion Stimulation Protein/metabolism; Fimbriae Proteins/chemistry; Fimbriae Proteins/genetics; Fimbriae Proteins/metabolism; Gene Expression Regulation, Bacterial; Genetic Loci; Genome, Bacterial; Integration Host Factors/metabolism; Molecular Chaperones/metabolism; Nucleic Acid Conformation; Operon; Protein Multimerization; Protein Structure, Tertiary; Recombinant Fusion Proteins/metabolism; Repressor Proteins/chemistry; Repressor Proteins/genetics; Repressor Proteins/metabolism |
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