PMID:2188964
| Citation |
Mei, BG and Zalkin, H (1990) Amino-terminal deletions define a glutamine amide transfer domain in glutamine phosphoribosylpyrophosphate amidotransferase and other PurF-type amidotransferases. J. Bacteriol. 172:3512-4 |
|---|---|
| Abstract |
A series of deletions was constructed in cloned Escherichia coli purF encoding glutamine phosphoribosylpyrophosphate amidotransferase. These deletions extended into the NH2 terminus of the protein and removed amino acids that are required for glutamine-dependent enzyme activity. Enzyme function, ascribed to the NH3-dependent activity, was retained in deletions that removed up to 237 amino acids. This result supports a model in which PurF-type amidotransferases contain an NH2-terminal glutamine amide transfer domain of approximately 194 to 200 amino acids fused to an aminator domain with NH3-dependent function. |
| Links | |
| Keywords |
Amidophosphoribosyltransferase/analysis; Amidophosphoribosyltransferase/genetics; Chromosome Deletion; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Glutamine/metabolism; Mutation; Pentosyltransferases/analysis; Structure-Activity Relationship |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.