PMID:2188952

Citation	Zhu, LQ, Gangopadhyay, T, Padmanabha, KP and Deutscher, MP (1990) Escherichia coli rna gene encoding RNase I: cloning, overexpression, subcellular distribution of the enzyme, and use of an rna deletion to identify additional RNases. J. Bacteriol. 172:3146-51
Abstract	The cloning and overexpression of the Escherichia coli rna gene encoding RNase I are described. Only a single copy of the rna gene is present on the E. coli chromosome. Although cells with as much as a 100-fold increase in RNase I activity were constructed, little effect on cell growth was observed. Overexpressed RNase I was found in the periplasmic space to the same degree (approximately 85%) as wild-type enzyme, suggesting no limitation in RNase I transport. The rna clone was used to identify a deletion strain totally lacking the rna gene. The normal growth of this strain showed that RNase I is not essential for cell viability. Extracts from the RNase I deletion strain still retained a low level of RNase activity in the presence of EDTA, conclusively demonstrating the existence of additional EDTA-active RNases in E. coli. The possibility of a RNase I inhibitor is also discussed.
Links	PubMed PMC209119
Keywords	Chromosome Deletion; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression; Genes, Bacterial; Ribonuclease, Pancreatic/analysis; Ribonuclease, Pancreatic/genetics
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