PMID:2185135

Citation	Malo, MS and Loughlin, RE (1990) Promoter elements and regulation of expression of the cysD gene of Escherichia coli K-12. Gene 87:127-31
Abstract	The cysD gene, involved in cysteine biosynthesis in Escherichia coli and Salmonella typhimurium, is positively regulated by the CysB regulatory protein. The cysD promoter of E. coli K-12 in a 492-bp PstI-Eco RI fragment was sequenced. The in vivo transcription start point (tsp) for the cysD gene was determined by the methods of T4 DNA polymerase mapping and mung-bean nuclease mapping. The -10 region of the cysD promoter (TATAGT) is closely homologous to the -10 consensus sequence (TATAAT) for E. coli promoters. The -35 region of this promoter (TTCATT) is less closely related to the -35 consensus sequence (TTGACA). Several mutants were obtained by using a chain-termination method for generating unidirectional deletions. Evidence is presented for a possible CysB protein binding site around -89, thought to be involved in regulation of expression of the cysD gene.
Links	PubMed
Keywords	Amino Acid Sequence; Base Sequence; Cystine/biosynthesis; Escherichia coli/genetics; Gene Expression Regulation, Bacterial; Genes, Bacterial; Genes, Regulator; Molecular Sequence Data; Oligonucleotide Probes; Promoter Regions, Genetic; Restriction Mapping; Salmonella typhimurium/genetics; Sequence Homology, Nucleic Acid; Transcription, Genetic
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