PMID:2183178
| Citation |
Lévêque, F, Plateau, P, Dessen, P and Blanquet, S (1990) Homology of lysS and lysU, the two Escherichia coli genes encoding distinct lysyl-tRNA synthetase species. Nucleic Acids Res. 18:305-12 |
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| Abstract |
In Escherichia coli, two distinct lysyl-tRNA synthetase species are encoded by two genes: the constitutive lysS gene and the thermoinducible lysU gene. These two genes have been isolated and sequenced. Their nucleotide and deduced amino acid sequences show 79% and 88% identity, respectively. Codon usage analysis indicates the lysS product being more efficiently translated than the lysU one. In addition, the lysS sequence exactly coincides with the sequence of herC, a gene which is part of the prfB-herC operon. In contrast to the recent proposal of Gampel and Tzagoloff (1989, Proc. Natl. Acad. Sci. USA 86, 6023-6027), the lysU sequence is distinct from the open reading frame located adjacent to frdA, although large homologies are shared by these two genes. |
| Links | |
| Keywords |
Amino Acid Sequence; Amino Acyl-tRNA Synthetases/genetics; Base Sequence; Cloning, Molecular; DNA Probes; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; DNA, Recombinant/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Library; Genes, Bacterial; Isoenzymes/genetics; Isoenzymes/isolation & purification; Lysine-tRNA Ligase/genetics; Lysine-tRNA Ligase/isolation & purification; Molecular Sequence Data; Plasmids; Restriction Mapping; Sequence Homology, Nucleic Acid |
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