PMID:2182126
| Citation |
Vladimirov, SN, Babkina, GT, Venijaminova, AG, Gimautdinova, OI, Zenkova, MA and Karpova, GG (1990) Structural arrangement of the decoding site of Escherichia coli ribosomes as revealed from the data on affinity labelling of ribosomes by analogs of mRNA--derivatives of oligoribonucleotides. Biochim. Biophys. Acta 1048:245-56 |
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| Abstract |
Using derivatives of oligoribonucleotides bearing an active group at the 5'- or 3'-end, the affinity modification of Escherichia coli ribosomes has been investigated in model complexes imitating various steps of initiation and elongation with a different extent of approximation to the real protein-synthesizing system. The protein environment of the ribosome decoding site is determined. The S3, S4, S9, L2, L7/L12 proteins belong to the 5'-region of the decoding site, and the S5, S7, S9, L1, L16 proteins to the 3'-region. In the process of translation the template moves along the external side of the 30 S subunit, from the L1 ridge to the L7/L12 stalk. The structural arrangement of the decoding site or its nearest environment depends on the functional state of ribosomes in the process of translation. |
| Links | |
| Keywords |
Affinity Labels/metabolism; Carbon Radioisotopes; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli/ultrastructure; Kinetics; Models, Structural; Oligoribonucleotides/chemical synthesis; Oligoribonucleotides/metabolism; Protein Biosynthesis; RNA, Messenger/metabolism; RNA, Transfer, Amino Acyl/metabolism; RNA, Transfer, Met; RNA, Transfer, Phe/metabolism; Ribosomal Proteins/isolation & purification; Ribosomal Proteins/metabolism; Ribosomes/metabolism; Ribosomes/ultrastructure |
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