PMID:2180957
| Citation |
Sharma, A, Henderson, BS, Schwab, JM and Smith, JL (1990) Crystallization and preliminary X-ray analysis of beta-hydroxydecanoyl thiol ester dehydrase from Escherichia coli. J. Biol. Chem. 265:5110-2 |
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| Abstract |
Escherichia coli beta-hydroxydecanoyl thiol ester dehydrase, a key enzyme for the biosynthesis of unsaturated fatty acids in E. coli, has been crystallized by the vapor diffusion method at pH 5.0-5.5 using 20% (w/v) polyethylene glycol (molecular weight 8000) as a precipitant. Two crystal forms have been characterized, and both diffract to at least 1.6 A. The orthorhombic crystals belong to space group P2(1)2(1)2(1), with cell constants of a = 68.4 A, b = 87.3 A, and c = 60.3 A. Monoclinic crystals are of space group C2, with a = 131.9 A, b = 71.5 A, c = 92.5 A, and beta = 103.5 degrees. |
| Links | |
| Keywords |
Crystallization; Escherichia coli/enzymology; Fatty Acid Synthetase Complex, Type II; Hydro-Lyases/isolation & purification; Protein Conformation; X-Ray Diffraction |
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