PMID:21803919
| Citation |
Abraham, S, Chin, J, Brouwers, HJ, Turner, B, Zhang, R and Chapman, TA (2011) Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins. Appl. Environ. Microbiol. 77:6691-3 |
|---|---|
| Abstract |
Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8). |
| Links |
PubMed PMC3187172 Online version:10.1128/AEM.00534-11 |
| Keywords |
Biosensing Techniques/methods; Colicins/toxicity; DNA Damage/drug effects; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/physiology; Gene Expression Regulation, Bacterial; Green Fluorescent Proteins/genetics; Green Fluorescent Proteins/metabolism; Promoter Regions, Genetic; SOS Response (Genetics); Transcription, Genetic |
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