PMID:2170325
| Citation |
Chen, J, Brevet, A, Fromant, M, Lévêque, F, Schmitter, JM, Blanquet, S and Plateau, P (1990) Pyrophosphatase is essential for growth of Escherichia coli. J. Bacteriol. 172:5686-9 |
|---|---|
| Abstract |
The ppa gene for inorganic pyrophosphatase is essential for the growth of Escherichia coli. A recombinant with a chromosomal ppa::Kanr lesion and a temperature-sensitive replicon with a ppa+ gene showed a temperature-sensitive growth phenotype, and a mutant with the sole ppa+ gene under control of the lac promoter showed inducer-dependent growth. When the lacp-ppa mutant was subcultured without inducer, the pyrophosphatase level decreased, the PPi level increased, and growth stopped. Cellular PPi reached 16 mM about 6 h after growth arrest without loss of cell viability. |
| Links | |
| Keywords |
Base Sequence; DNA, Bacterial/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/growth & development; Genes, Bacterial; Genotype; Inorganic Pyrophosphatase; Molecular Sequence Data; Oligonucleotide Probes; Phenotype; Plasmids; Pyrophosphatases/genetics; Pyrophosphatases/metabolism; Replicon; Restriction Mapping; Temperature |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.