PMID:21696605
| Citation |
Derous, V, Deboeck, F, Hernalsteens, JP and De Greve, H (2011) Reproducible gene targeting in recalcitrant Escherichia coli isolates. BMC Res Notes 4:213 |
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| Abstract |
A number of allele replacement methods can be used to mutate bacterial genes. For instance, the Red recombinase system of phage Lambda has been used very efficiently to inactivate chromosomal genes in E. coli K-12, through recombination between regions of homology. However, this method does not work reproducibly in some clinical E. coli isolates. |
| Links |
PubMed PMC3146853 Online version:10.1186/1756-0500-4-213 |
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