PMID:2166131
| Citation |
Delaney, JM (1990) A grpE mutant of Escherichia coli is more resistant to heat than the wild-type. J. Gen. Microbiol. 136:797-801 |
|---|---|
| Abstract |
The grpE gene of Escherichia coli is essential for bacteriophage lambda DNA replication and is also necessary for host RNA and DNA synthesis at high temperature. A grpE mutant of E. coli was found to be substantially more resistant to 50 degrees C heat treatment than the wild-type. Upon receiving a 42 degrees C heat shock for 15 min, both the wild-type and the grpE mutant became more resistant to heat (i.e. they became thermotolerant). A grpE+ revertant behaved similarly to the wild-type in that it was more sensitive to heat than grpE cells. In addition, grpE cells had the same H2O2 and UV sensitivity as the wild-type. This implies that the conditions for which a grpE mutation is beneficial are unique to heat exposure and are not caused by H2O2 or UV exposure. Furthermore, synthesis of heat-shock proteins occurred sooner in the grpE mutant than in the wild-type, indicating that the grpE gene of E. coli may influence the regulation of the heat-shock response. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacteriophage lambda/genetics; DNA Replication/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/genetics; Hot Temperature; Mutation |
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