PMID:2165018
| Citation |
Quirk, S, Bhatnagar, SK and Bessman, MJ (1990) Primary structure of the deoxyguanosine triphosphate triphosphohydrolase-encoding gene (dgt) of Escherichia coli. Gene 89:13-8 |
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| Abstract |
The complete nucleotide sequence has been determined for a 2027-bp region that encompasses the structural gene (dgt) encoding deoxyguanosine triphosphate triphosphohydrolase (dGTPase) from Escherichia coli. The gene resides between the htrA and dapD loci at 3.75-3.8' on the bacterial chromosome. Using homologous recombination in a recD recipient, a dgt- bacterial strain was constructed that was deficient in producing functional dGTPase. Comparison of dGTP pools in this and other strains revealed that dGTPase synthesized in vivo does to some degree modulate the level of dGTP in the bacterial cell, yet the magnitude of this modulation may be insufficient to explain the physiological function of dGTPase. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Chromosome Mapping; Chromosomes, Bacterial; Cloning, Molecular/methods; Deoxyguanine Nucleotides/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Molecular Sequence Data; Phosphoric Monoester Hydrolases/genetics; Plasmids; Restriction Mapping; Sequence Homology, Nucleic Acid |
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