PMID:2164680
| Citation |
Umezu, K, Nakayama, K and Nakayama, H (1990) Escherichia coli RecQ protein is a DNA helicase. Proc. Natl. Acad. Sci. U.S.A. 87:5363-7 |
|---|---|
| Abstract |
The Escherichia coli recQ gene, a member of the RecF recombination gene family, was set in an overexpression plasmid, and its product was purified to near-homogeneity. The purified RecQ protein exhibited a DNA-dependent ATPase and a helicase activity. Without DNA, no ATPase activity was detected. The capacity as ATPase cofactor varied with the type of DNA in the following order: circular single strand greater than linear single strand much greater than circular or linear duplex. As a helicase, RecQ protein displaced an annealed 71-base or 143-base single-stranded fragment from circular or linear phage M13 DNA, and the direction of unwinding seemed to be 3'----5' with respect to the DNA single strand to which the enzyme supposedly bound. Furthermore, the protein could unwind 143-base-pair blunt-ended duplex DNA at a higher enzyme concentration. It is concluded that RecQ protein is a previously unreported helicase, which might possibly serve to generate single-stranded tails for a strand transfer reaction in the process of recombination. |
| Links | |
| Keywords |
Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/isolation & purification; Adenosine Triphosphatases/metabolism; Base Sequence; DNA Helicases/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Kinetics; Molecular Sequence Data; Oligonucleotide Probes; Plasmids; RecQ Helicases; Recombinant Fusion Proteins/isolation & purification; Recombinant Fusion Proteins/metabolism; Restriction Mapping |
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