PMID:21601084
| Citation |
An, W and Chin, JW (2011) Orthogonal gene expression in Escherichia coli. Meth. Enzymol. 497:115-34 |
|---|---|
| Abstract |
Here, we describe a route orthogonal gene expression which combines orthogonal transcription and translation using library-based selections. We show how orthogonal gene expression can be used to create a minimal orthogonal ribosome and describe how to create orthogonal transcription-translation feed forward loops that introduce tailored information processing delays into gene expression. |
| Links |
PubMed Online version:10.1016/B978-0-12-385075-1.00005-6 |
| Keywords |
DNA-Directed RNA Polymerases/genetics; DNA-Directed RNA Polymerases/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Gene Expression Regulation, Bacterial; Gene Library; Nucleic Acid Conformation; Promoter Regions, Genetic; Protein Biosynthesis; RNA, Messenger/chemistry; RNA, Messenger/genetics; RNA, Messenger/metabolism; Ribosomes/genetics; Ribosomes/metabolism; Transcription, Genetic; Viral Proteins/genetics; Viral Proteins/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.