PMID:21596992
| Citation |
Dunkle, JA, Wang, L, Feldman, MB, Pulk, A, Chen, VB, Kapral, GJ, Noeske, J, Richardson, JS, Blanchard, SC and Cate, JH (2011) Structures of the bacterial ribosome in classical and hybrid states of tRNA binding. Science 332:981-4 |
|---|---|
| Abstract |
During protein synthesis, the ribosome controls the movement of tRNA and mRNA by means of large-scale structural rearrangements. We describe structures of the intact bacterial ribosome from Escherichia coli that reveal how the ribosome binds tRNA in two functionally distinct states, determined to a resolution of ~3.2 angstroms by means of x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit site. The structures help to explain how the ratchet-like motion of the two ribosomal subunits contributes to the mechanisms of translocation, termination, and ribosome recycling. |
| Links |
PubMed PMC3176341 Online version:10.1126/science.1202692 |
| Keywords |
Anticodon/chemistry; Anticodon/metabolism; Crystallography, X-Ray; Escherichia coli; Escherichia coli Proteins/metabolism; Models, Molecular; Nucleic Acid Conformation; Protein Biosynthesis; RNA, Bacterial/chemistry; RNA, Bacterial/metabolism; RNA, Messenger/chemistry; RNA, Messenger/metabolism; RNA, Ribosomal, 16S/chemistry; RNA, Ribosomal, 16S/metabolism; RNA, Ribosomal, 23S/chemistry; RNA, Ribosomal, 23S/metabolism; RNA, Transfer, Amino Acyl/chemistry; RNA, Transfer, Amino Acyl/metabolism; RNA, Transfer, Phe/chemistry; RNA, Transfer, Phe/metabolism; Ribosomal Proteins/metabolism; Ribosome Subunits, Large, Bacterial/chemistry; Ribosome Subunits, Large, Bacterial/metabolism; Ribosome Subunits, Large, Bacterial/ultrastructure; Ribosome Subunits, Small, Bacterial/chemistry; Ribosome Subunits, Small, Bacterial/metabolism; Ribosome Subunits, Small, Bacterial/ultrastructure |
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