PMID:21592194
| Citation |
Rodas, PI, Trombert, AN and Mora, GC (2011) A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi. FEMS Microbiol. Lett. 321:58-66 |
|---|---|
| Abstract |
We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1(™) single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope. |
| Links |
PubMed Online version:10.1111/j.1574-6968.2011.02310.x |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Cell Wall/genetics; Cell Wall/metabolism; Computational Biology; Gene Expression Regulation, Bacterial; Gene Order; Molecular Sequence Data; Open Reading Frames/genetics; Salmonella typhi/genetics; Salmonella typhi/growth & development; Salmonella typhi/metabolism; Sequence Alignment |
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