PMID:21532574
| Citation |
So, LH, Ghosh, A, Zong, C, Sepúlveda, LA, Segev, R and Golding, I (2011) General properties of transcriptional time series in Escherichia coli. Nat. Genet. 43:554-60 |
|---|---|
| Abstract |
Gene activity is described by the time series of discrete, stochastic mRNA production events. This transcriptional time series shows intermittent, bursty behavior. One consequence of this temporal intricacy is that gene expression can be tuned by varying different features of the time series. Here we quantify copy-number statistics of mRNA from 20 Escherichia coli promoters using single-molecule fluorescence in situ hybridization in order to characterize the general properties of these transcriptional time series. We find that the degree of burstiness is correlated with gene expression level but is largely independent of other parameters of gene regulation. The observed behavior can be explained by the underlying variation in the duration of bursting events. Using Shannon's mutual information function, we estimate the mutual information transmitted between an outside stimulus, such as the extracellular concentration of inducer molecules, and intracellular levels of mRNA. This suggests that the outside stimulus transmits information reflected in the properties of transcriptional time series. |
| Links |
PubMed PMC3102781 Online version:10.1038/ng.821 |
| Keywords |
Escherichia coli/genetics; Gene Dosage; Gene Expression Regulation; In Situ Hybridization, Fluorescence; Kinetics; RNA, Messenger/biosynthesis; RNA, Messenger/metabolism; Time; Transcription, Genetic |
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