PMID:21527678
| Citation |
Boal, AK, Grove, TL, McLaughlin, MI, Yennawar, NH, Booker, SJ and Rosenzweig, AC (2011) Structural basis for methyl transfer by a radical SAM enzyme. Science 332:1089-92 |
|---|---|
| Abstract |
The radical S-adenosyl-L-methionine (SAM) enzymes RlmN and Cfr methylate 23S ribosomal RNA, modifying the C2 or C8 position of adenosine 2503. The methyl groups are installed by a two-step sequence involving initial methylation of a conserved Cys residue (RlmN Cys(355)) by SAM. Methyl transfer to the substrate requires reductive cleavage of a second equivalent of SAM. Crystal structures of RlmN and RlmN with SAM show that a single molecule of SAM coordinates the [4Fe-4S] cluster. Residue Cys(355) is S-methylated and located proximal to the SAM methyl group, suggesting the SAM that is involved in the initial methyl transfer binds at the same site. Thus, RlmN accomplishes its complex reaction with structural economy, harnessing the two most important reactivities of SAM within a single site. |
| Links |
PubMed PMC3506250 Online version:10.1126/science.1205358 |
| Keywords |
Bacterial Proteins/chemistry; Bacterial Proteins/metabolism; Catalytic Domain; Crystallography, X-Ray; Cysteine/chemistry; Escherichia coli/enzymology; Escherichia coli/growth & development; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/metabolism; Evolution, Molecular; Hydrogen Bonding; Methylation; Methyltransferases/chemistry; Methyltransferases/metabolism; Models, Molecular; Oxidation-Reduction; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; RNA, Bacterial/metabolism; RNA, Ribosomal, 23S/metabolism; S-Adenosylmethionine/chemistry; S-Adenosylmethionine/metabolism |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.