PMID:21486250
| Citation |
Smith, HE (2011) Identifying insertion mutations by whole-genome sequencing. BioTechniques 50:96-7 |
|---|---|
| Abstract |
Insertion mutagenesis via mobile genetic element is a common technique for the analysis of gene function in model organisms. Next-generation sequencing offers an attractive approach for localizing the site of insertion, but alignment-based mapping of mobile genetic elements is challenging. A computational method for identifying insertion sites is reported herein. The technique was validated by mapping transposons in both bacterial and nematode species. The approach should be extensible to other systems that employ mobile genetic elements to generate mutations. |
| Links |
PubMed Online version:10.2144/000113600 |
| Keywords |
Animals; Caenorhabditis elegans/genetics; DNA/genetics; DNA Transposable Elements; DNA, Bacterial/genetics; Escherichia coli/genetics; Genome; Genome, Bacterial; Mutagenesis, Insertional; Sequence Alignment/methods; Sequence Analysis, DNA/methods |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.