PMID:21485854
| Citation |
Singh, G, Vajpayee, P, Khatoon, I, Jyoti, A, Dhawan, A, Gupta, KC and Shanker, R (2011) Chromium oxide nano-particles induce stress in bacteria: probing cell viability. J Biomed Nanotechnol 7:166-7 |
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| Abstract |
In this study, viability of an environmentally relevant bacterium, Escherichia coli exposed to 0-100 microg/mL chromium oxide nanoparticles (Cr2O3, Nps) for 120 min in Luria Bertani broth was evaluated by Propidium monoazide (PMA) assisted Q-PCR and standard plate count (SPC) method. Viable count for E. coli grown in Cr2O3, Nps amended medium was more by PMA assisted Q-PCR than SPC. Thus, the observations made in this study suggest that the inclusion of PMA assisted Q-PCR for viability assessment in Nps toxicity studies will provide the real count for the viable cells comprising of both viable and viable but not culturable (VBNC) cells. |
| Links | |
| Keywords |
Cell Survival/drug effects; Chromium Compounds/pharmacology; Dose-Response Relationship, Drug; Escherichia coli/drug effects; Escherichia coli/physiology; Metal Nanoparticles/administration & dosage; Oxidative Stress/drug effects |
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