PMID:2144836
| Citation |
Bonthron, DT (1990) L-asparaginase II of Escherichia coli K-12: cloning, mapping and sequencing of the ansB gene. Gene 91:101-5 |
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| Abstract |
The Escherichia coli gene ansB, encoding the chemotherapeutic enzyme L-asparaginase II, has been cloned, using a strategy based on the polymerase chain reaction, and sequenced. The amino acid (aa) sequence differs in eleven positions from the data previously derived by direct aa sequencing. A cleavable secretory signal peptide precedes the N terminus of the mature protein. The ansB gene maps to position 3114 kb on the physical map of E. coli [Kohara et al., Cell 50 (1987) 495-508], corresponding to approx. 63.8 min on the genetic map. |
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| Keywords |
Amino Acid Sequence; Asparaginase/genetics; Bacteriophage lambda/genetics; Base Sequence; Chromosomes, Bacterial; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Isoenzymes/genetics; Molecular Sequence Data; Oligonucleotide Probes; Protein Sorting Signals/genetics; Restriction Mapping |
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