PMID:21435272
| Citation |
Minty, JJ, Lesnefsky, AA, Lin, F, Chen, Y, Zaroff, TA, Veloso, AB, Xie, B, McConnell, CA, Ward, RJ, Schwartz, DR, Rouillard, JM, Gao, Y, Gulari, E and Lin, XN (2011) Evolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli. Microb. Cell Fact. 10:18 |
|---|---|
| Abstract |
Isobutanol is a promising next-generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titer. Organic solvent tolerance is a complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing and a gene expression study to elucidate genetic bases of adaptation to exogenous isobutanol stress. |
| Links |
PubMed PMC3071312 Online version:10.1186/1475-2859-10-18 |
| Keywords |
Butanols/toxicity; Drug Tolerance; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins/chemistry; Escherichia coli Proteins/genetics; Escherichia coli Proteins/metabolism; Evolution, Molecular; Gene Expression Regulation, Bacterial; Genome, Bacterial; Genotype; Lipoproteins/chemistry; Lipoproteins/genetics; Lipoproteins/metabolism; Membrane Transport Proteins/chemistry; Membrane Transport Proteins/genetics; Membrane Transport Proteins/metabolism; Multidrug Resistance-Associated Proteins/chemistry; Multidrug Resistance-Associated Proteins/genetics; Multidrug Resistance-Associated Proteins/metabolism; Mutation; Oligonucleotide Array Sequence Analysis; Phenotype; Sequence Analysis, DNA |
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