PMID:21431742

Citation	Borogovac, A and Broude, NE (2011) Visualization of induced RNA in single bacterial cells. Methods Mol. Biol. 714:189-99
Abstract	Visualization of RNA in live cells is a challenging task due to the transient character of most RNA molecules and the lack of adequate methods to label RNA noninvasively. Here, we describe a system for regulated RNA synthesis and visualization of RNA in live Escherichia coli cells based on protein complementation. This method allows for labeling RNA with a relatively small protein complex that becomes fluorescent only when bound to an RNA. This method greatly reduces the high fluorescence background characteristic of methods employing intact fluorescent proteins. A short reporter RNA was shown to localize at the cell periphery in nonrandom patterns.
Links	PubMed Online version:10.1007/978-1-61779-005-8_12
Keywords	Aptamers, Nucleotide/metabolism; Cells, Immobilized/cytology; Cells, Immobilized/metabolism; Escherichia coli/cytology; Escherichia coli/genetics; Escherichia coli/metabolism; Flow Cytometry; Genes, Reporter/genetics; Glass/chemistry; Kinetics; Microscopy, Fluorescence; Molecular Imaging/methods; Plasmids/genetics; Protein Biosynthesis; RNA, Bacterial/analysis; RNA, Bacterial/biosynthesis; RNA, Bacterial/chemistry; RNA, Bacterial/genetics; Single-Cell Analysis/methods; Surface Properties; Transcription, Genetic
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