PMID:21429210
| Citation |
Scheidle, M, Dittrich, B, Klinger, J, Ikeda, H, Klee, D and Büchs, J (2011) Controlling pH in shake flasks using polymer-based controlled-release discs with pre-determined release kinetics. BMC Biotechnol. 11:25 |
|---|---|
| Abstract |
There are significant differences in the culture conditions between small-scale screenings and large-scale fermentation processes. Production processes are usually conducted in fed-batch cultivation mode with active pH-monitoring and control. In contrast, screening experiments in shake flasks are usually conducted in batch mode without active pH-control, but with high buffer concentrations to prevent excessive pH-drifts. These differences make it difficult to compare results from screening experiments and laboratory and technical scale cultivations and, thus, complicate rational process development. In particular, the pH-value plays an important role in fermentation processes due to the narrow physiological or optimal pH-range of microorganisms. To reduce the differences between the scales and to establish a pH-control in shake flasks, a newly developed easy to use polymer-based controlled-release system is presented in this paper. This system consists of bio-compatible silicone discs embedding the alkaline reagent Na2CO3. Since the sodium carbonate is gradually released from the discs in pre-determined kinetics, it will ultimately compensate the decrease in pH caused by the biological activity of microorganisms. |
| Links |
PubMed PMC3076237 Online version:10.1186/1472-6750-11-25 |
| Keywords |
Bacteriological Techniques/instrumentation; Bacteriological Techniques/methods; Carbonates/metabolism; Carbonates/pharmacokinetics; Culture Media/chemistry; Culture Media/metabolism; Escherichia coli/growth & development; Escherichia coli/metabolism; Fermentation; Hydrogen-Ion Concentration; Kinetics; Polymers/metabolism; Reproducibility of Results |
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