PMID:21414195

Citation	Huber, R, Roth, S, Rahmen, N and Büchs, J (2011) Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation. BMC Biotechnol. 11:22
Abstract	The specific productivity of cultivation processes can be optimized, amongst others, by using genetic engineering of strains, choice of suitable host/vector systems or process optimization (e.g. choosing the right induction time). A further possibility is to reduce biomass buildup in favor of an enhanced product formation, e.g. by limiting secondary substrates in the medium, such as phosphate. However, with conventional techniques (e.g. small scale cultivations in shake flasks), it is very tedious to establish optimal conditions for cell growth and protein expression, as the start of protein expression (induction time) and the degree of phosphate limitation have to be determined in numerous concerted, manually conducted experiments.
Links	PubMed PMC3068942 Online version:10.1186/1472-6750-11-22
Keywords	Culture Media/metabolism; Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/metabolism; Gene Expression; Gene Expression Regulation, Bacterial; Genetic Engineering; High-Throughput Screening Assays/instrumentation; High-Throughput Screening Assays/methods; Phosphates/metabolism; Recombinant Proteins/genetics; Recombinant Proteins/metabolism
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